Spain thumped Croatia 6-0 in the reverse match back in September and know that another victory would move them onto nine points from their four Group 4 fixtures, which would be enough to top the section.
La Roja did suffer a 3-2 home defeat to England in their last UEFA Nations League match, however, and there is no question that Croatia have the quality to overcome Luis Enrique's side.
Thursday will actually be just the eighth meeting between these two international teams, with Spain winning four of the previous seven.
Here, Sports Mole takes a look at how Spain could line up in Zagreb.
Spain boss Enrique shocked many when he recalled Jordi Alba to his latest squad. The pair do not have the best of relationships, but Alba's form for Barcelona this season has been excellent, and the full-back is expected to be given the nod on Thursday night.