Both players sustained the complaints during Northern Ireland's 2-1 loss at home to Estonia yesterday evening.
"Lee Camp and Grant McCann have been ruled out of Northern Ireland's final Euro 2012 qualifier against Italy on Tuesday," confirmed a statement on the NI Football Association's website.
"The pair will miss the game as a result of groin injuries."
Coventry City midfielder Sammy Clingan has been rated doubtful for the meeting with Italy because of a back injury.
"Sammy's back went into spasm," said manager Nigel Worthington after last night's defeat. "That's not too clever and he's one of the ones we'll be looking at over the next 24 hours."
Worthington is also without Jonny Evans, who has an ankle problem.