The 30-goal striker picked up an injury against Besiktas in the last round of the competition, but he managed 60 minutes in the 3-2 win over Nantes last time out and is picked for a second consecutive start.
Bruno Genesio has made four changes as the Ligue 1 side bid to overturn a 4-1 aggregate deficit, with Mouctar Diakhaby, Corentin Tolisso, Mathieu Valbuena and Maxwel Cornet also drafted in from the off.
The visitors, meanwhile, have made six changes ahead of their visit to the Parc Olympique Lyonnais, with Matthijs de Ligt, Davinson Sanchez, Hakim Ziyech, Lasse Schone, Bertrand Traore and Amin Younes all back in the XI.
Lyon: Lopes; Rafael, N'Koulou, Diakhaby, Morel; Gonalons, Tolisso; Cornet, Fekir, Valbuena; Lacazette
Subs: Darder, Ferri, Ghezzal, Gorgelin, Jallet, Rybus, Tousart
Ajax: Onana; Veltman, Sanchez, De Ligt, Viergever; Klaassen, Shone, Ziyech; Traore, Dolberg, Younes
Subs: Boer, Kluivert, Neres, Riedwald, Tete, Van de Beek, Westermann
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