In contrast, Hibernian's campaign started in perfect fashion as they enjoyed a 2-1 victory over Kilmarnock on Saturday.
Having managed to navigate three pre-season fixtures without defeat, Livingston fans would have been forgiven for expecting their team to triumph over a St Mirren side who finished the 2019-20 campaign in the bottom half of the table.
While it is certainly not the ideal start for Livingston, who registered a top-five finish last season, there is still plenty of time to put things right in their third successive season of top-flight football.
Livi are now returning to familiar territory at the Almondvale Stadium, where they have triumphed in six of their last eight Scottish Premiership outings and are yet to taste defeat on home turf since the turn of the year.
Meanwhile, Kilmarnock were unable to storm a late comeback against Hibernian as Jack Ross witnessed his side come away with all three points on the first day of the season.
Martin Boyle opened his account for the campaign after only five minutes before bagging his second just after the half-hour mark, and even though Kilmarnock's Chris Burke scored a thunderous free-kick to set up a nervy second half, Hibs held on to take the spoils.
Having just missed out on a top-six finish as the 2019-20 season was decided on a points-per-game basis, Hibs will no doubt be motivated to push for a European berth this time around.
Ross's men will now face a Livingston side whom they have beaten 14 times from 21 outings, but the two clubs played out 2-2 and 1-1 draws either side of a 2-0 Livingston victory last season.
Livingston Scottish Premiership form: L
Hibernian Scottish Premiership form: W
Former captain Alan Lithgow is Livingston's only long-term absentee after undergoing hip surgery, with Holt otherwise having a full squad of players at his disposal.
Livingston's players would have no doubt felt frustrated as they walked away from their encounter with St Mirren empty-handed, and Holt may be reluctant to make too many changes to his side despite their opening-day defeat.
As far as Hibs are concerned, Ross is expected to stick with the majority of the same side that got the job done over Kilmarnock on the opening day.
Christian Doidge had to make do with a spot among the replacements last weekend, but with Drey Wright struggling with a thigh strain, Boyle may start on the right-hand side allowing Doidge to partner Kevin Nisbet up top.
Livingston possible starting lineup:
McCrorie; Fitzwater, Guthrie, Taylor-Sinclair; Devlin, Bartley, Robinson, Ambrose; Forrest, Souda; Dykes
Hibernian possible starting lineup:
Marciano; McGinn, Porteous, Hanlon; Boyle, Allan, Gogic, Newell, Doig; Nisbet, Doidge
We say: Livingston 1-1 Hibernian
This is a difficult game to call. Both sides will be eyeing up a top-four finish this season, and even though Livingston failed to triumph on the opening day, Holt's men did not perform all that badly whatsoever. Hibs should feel confident of taking all three points following their recent win, but Livi boast a strong recent home record, so we expect the two clubs to play out a closely-fought draw on Saturday afternoon.