The Saints on Thursday announced that Hasenhuttl had been cleared to end his period of self-isolation, which followed his wife receiving a positive result to a Covid-19 test and meant he was not in attendance for the 0-0 draw with West Ham on Tuesday.
Liverpool manager Jurgen Klopp looks set to freshen things up after a performance lacking sharpness at Newcastle.
Thiago Alcantara made his long-awaited comeback in the 0-0 draw after more than two months out with a knee injury and could be in line for only his second start of the season with Georginio Wijnaldum set to return to the line-up after being rested.
Fellow midfielder Xherdan Shaqiri also returned in that game and could get a longer run out, while centre-back Nat Phillips looks set to keep his place with Joel Matip out for three weeks with a groin problem.
Southampton provisional squad: McCarthy, Forster, Walker-Peters, Bertrand, Stephens, Valery, Salisu, Vokins, Bednarek, Romeu, Ward-Prowse, Djenepo, Armstrong, Smallbone, Diallo, Walcott, Long, Ings, Adams, Obafemi, N'Lundulu.
Liverpool provisional squad: Alisson, Alexander-Arnold, Fabinho, Phillips, Robertson, Wijnaldum, Thiago, Oxlade-Chamberlain, Salah, Firmino, Mane, Kelleher, Adrian, N Williams, R Williams, Milner, Henderson, Jones, Minamino, Origi.