The 34-year-old, who has helped fire the Londoners to the summit of League One with eight goals, was thought to be a doubt for the clash with Karl Robinson's men after sustaining a dead leg in last week's 1-1 draw with Oldham Athletic.
However, the veteran striker appears to have shrugged off the knock which threatened his inclusion and will line up against the Dons as the O's look to extend their unbeaten run to 11 games.
Lisbie will start alongside David Mooney as the spearhead of Orient's attack, with the in-form duo hitting 16 goals between them so far this season.
Meanwhile, MK Dons manager Robinson has named three changes from the 3-2 defeat to Gillingham last week.
Leyton Orient: Jones; Omozusi, Vincelot, Cuthbert, Baudry, Cox, James, Odubajo, Clark; Lisbie, Mooney
MK Dons: McLoughlin; Otsemobor, Flanagan, Williams, Lewington; Potter, Gleeson; Powell, Chadwick, Carruthers; Bamford