The defeat was the club's third match without a victory and Warnock will be hoping his side can recapture the spirit that saw them beat Everton 2-1 in the last round.
Frazer Richardson and Jack Cork could also return after thigh and knee injuries, with manager Nigel Adkins likely to make several changes with his side taking to the pitch at Elland Road just over 48 hours after they lost 2-1 to Tottenham Hotspur.
Possible starting lineups:
Leeds: Kenny; Peltier, Lees, Pearce, Byram; Austin, Pugh, Tonge, Norris; Becchio, Diouf
Southampton: Davis; Yoshida, Hooiveld, Richardson, Seaborne; Ward-Prowse, Cork, Guly, Chaplow, De Ridder; Lee
Sports Mole says: 1-2