In all, manager Gareth Southgate makes four changes to the starting XI that suffered a 1-0 defeat in Germany on Wednesday, with the 34-year-old Sunderland striker coming in for Jamie Vardy, who begins among the substitutes.
The Three Lions are expected to switch to four at the back for the clash in the capital, with Raheem Sterling also earning a recall as support for Defoe alongside Dele Alli and Adam Lallana in a 4-2-3-1 formation.
Jake Livermore drops to the bench as Alex Oxlade-Chamberlain partners up with Eric Dier, while Michael Keane is joined in the heart of defence by John Stones, with Kyle Walker coming in on the right and Ryan Bertrand dropping to left-back ahead of Joe Hart between the sticks.
For the visitors, manager Edgaras Jankauskas makes five changes from the aside that suffered a 3-0 friendly defeat in the Czech Republic in midweek, with Nerijus Valskis picked to lead the line and Arturas Zulpa, skipper Fiodor Cernych, Arvydas Novikovas and Tadas Kijanskas also earning recalls.
England: Hart; Walker, Stones, Keane, Bertrand; Dier, Oxlade-Chamberlain; Sterling, Alli, Lallana; Defoe
Subs: Clyne, Forster, Shaw, Gibson, Livermore, Ward-Prowse, Barkley, Vardy, Rashford, Lingard, Redmond, Heaton
Lithuania: Setkus; Slavickas, Klimavicius, Kijanskas, Vaitkunas; Zulpa, Kuklys; Cernych, Slivka, Novikovas; Valskis
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