The left-back was cautioned for the second time this summer during their final game of the group stage - a 1-0 defeat to England - and will take his place on the naughty step for the weekend's game at the Puskas Arena.
The only other dilemma for the Czech coach is in midfield, as Alex Kral continues to push for a start in midfield alongside Tomas Soucek, but Silhavy could keep faith in Tomas Holes from the first whistle.
An unchanged attack would see Lukas Masopust, Vladimir Darida and Jakub Jankto feeding Patrik Schick - who boasts three goals for the tournament so far - but he only managed one shot on target during his country's final two group games.
Darida has now emerged as a fresh doubt due to an injury picked up in training, but Silhavy has not ruled the Hertha Berlin playmaker out of the contest.
Czech Republic possible starting lineup: Vaclik; Coufal, Celustka, Kalas, Mateju; Holes, Soucek; Masopust, Darida, Jankto; Schick