Diego Simeone's side will enter the match off the back of a 1-0 loss to Juventus in the Champions League on Tuesday night, while they were held to a 1-1 draw at Granada in the league last weekend.
Atletico have actually shared the points in seven of their 14 La Liga outings this season, although they are fourth in the table, just three points off leaders Barca.
Here, Sports Mole looks at how the home side could line up in the capital.
Atletico will again be without the services of key central defender Stefan Savic due to a thigh problem, while experienced striker Diego Costa is out until at least February after undergoing a neck operation.
Sime Vrsaljko is also unavailable as he battles to recover from knee surgery, but Jose Gimenez was pictured in training earlier this week ahead of the clash with Barca.
Saul Niguez and Thomas Partey were both suspended for the clash with Granada last weekend but are now available, while Joao Felix stepped up his recovery from an ankle injury by appearing against Juve.