Both sides are competing in this competition for a second time, albeit in different circumstances.
The Gunners will see this as a step down after finishing a lowly sixth in the Premier League last season, while Vorskla's reward for a third-placed finish in the Ukranian top flight is a spot in UEFA's secondary competition.
Follow all of the action as it unfolds in North London with our extensive updates below.
STARTING XI: Leno; Lichtsteiner, Sokratis, Holding, Monreal; Elneny, Torreira; Welbeck, Mkhitaryan, Iwobi; Aubameyang
SUBS: Martinez, Bellerin, Lacazette, Ozil, Guendouzi, Nketiah, Smith Rowe
STARTING XI: Shust; Perduta, Chesnakov, Dallku, Artur; Kobakhidze, Sharpar, Kravchenko, Rebenok; Kolomoets; Kulach
SUBS: Tkachenko, Girogadze, Sakiv, Misik, Sklyar, Careca, Sergiychuk
Unai Emery: "The Premier League is the first competition because it's giving us a big responsibility. Other competitions are very important too. Today, we are only thinking about giving our supporters the first performance and a good 90 minutes on the pitch at the Emirates. Every title for us is very important. In my career, the ambition is very important for continuing to improve and continuing to achieve the objectives in my career personally and with the team. I want to play for every title going forward."
Vasyl Sachko: "We have a planned stroll for the team. We are not here as tourists, we are here to play, but we will have a walk around tomorrow as part of our warm-up. I have never been here as a fan, I haven't been here once but the impressions are the best they could be. Our club is writing history, writing history for our city and that is a huge honour for us. Our objective is to get the players to understand this. Our team is playing against Arsenal, we understand we are a modest club. We don't have any stars in our team."
ARSENAL SUBS: Martinez, Bellerin, Lacazette, Ozil, Guendouzi, Nketiah, Smith Rowe
VORSKLA POLTAVA SUBS: Tkachenko, Girogadze, Sakiv, Misik, Sklyar, Careca, Sergiychuk