Berwick shocked Scottish League Two leaders Arbroath as goals from Lee Currie, Paul Willis and Andrew Russell gave them a 3-1 win at Shielfield Park.
However, Allan Moore's side stay top as second-placed Albion Rovers were beaten 2-0 by Clyde.
Steven McDougall and Scott Ferguson scored the goals that denied Albion pole position, while Queen's Park also lost to complete a miserable weekend for the top three.
Annan Athletic's Josh Todd and Peter Weatherson were on target for the Galabankies, but they remain a long way adrift of the the top three despite their respective defeats.
At the bottom of the table, East Stirlingshire recorded a 1-0 win over Elgin City to move to within one point of ninth-placed Montrose, who were soundly beaten by East Fife as goals from Sean Dickson, Ross Brown and Fraser Mullen gave Gary Naysmith's side a comfortable win.
Results in full: Albion Rovers 0-2 Clyde, Annan Athletic 2-0 Queen's Park, Berwick 3-1 Arbroath, East Stirlingshire 1-0 Elgin City, Montrose 0-3 East Fife.
