Last weekend's 5-3 reversal at fellow strugglers Bristol City was the club's first defeat in six. The result leaves them two points ahead of the bottom three with a game in hand on 22nd-placed Wolverhampton Wanderers.
Bolton will be looking for three points to keep alive their slim hopes of reaching the playoffs, with Dougie Freedman's side currently seven points adrift of sixth place.
Barnsley boss David Flitcroft will be without defender Scott Golbourne, who has undergone surgery on a knee injury. On-loan Manchester United midfielder Ryan Tunnicliffe is expected to retain his place in the starting XI for the game in South Yorkshire.
Bolton Wanderers: LWDDWW
Possible starting lineups
Barnsley: Steele; Foster, O'Brien, Perkins, McNulty; Cranie, Delap, Tunnicliffe, Kennedy; Harewood, Dagnall
Bolton Wanderers: Bogdan; Alonso, Knight, Dawson, Ricketts; Davies, Spearing, Pratley, Lee; Sordell, Ngog
Sports Mole says: 1-1