The social networking site has caused a number of controversies in sport since its introduction, but Olazabal has refused to issue a complete ban on the site.
Instead, the 46-year-old has called for caution when using the networking tool, warning his players that any comments could be misconstrued or taken out of context.
"I'm not banning any member of the team," said Olazabal.
"What I've said to them is that they have to be careful on what they say, how they say it and when they say it. I'm sure that any comments that are made on Twitter are not intended in a bad way, but if you take the sentence out of context, it might look completely different.
"In that regard, the boys need to be a little bit cautious about it."
The Ryder Cup gets underway on Friday with Europe looking to win it for the fifth time in the last six meetings.