Luke Donald will take on Bubba Watson in the opening singles match on the final day of the Ryder Cup on Sunday.
The 34-year-old Englishman will be in charge of kickstarting Europe's comeback against the United States, who currently have a 10-6 lead.
Ian Poulter will be second out against Webb Simpson, and the pair will be followed by the battle between Keegan Bradley and Rory McIlroy.
World number two Tiger Woods will be the last man out for the USA, and he will go head-to-head against Francesco Molinari.
Europe need to score eight points from the 12 singles to reach 14 points and retain the Ryder Cup.
As for the US, they need to collect four and-a-half points to win the competition.
Sunday's full draw:
Bubba Watson v Luke Donald Webb Simpson v Ian Poulter Keegan Bradley v Rory McIlroy Phil Mickelson v Justin Rose Brandt Snedeker v Paul Lawrie Dustin Johnson v Nicolas Colsaerts Zach Johnson v Graeme McDowell Jim Furyk v Sergio Garcia Jason Dufner v Peter Hanson Matt Kuchar v Lee Westwood Steve Stricker v Martin Kaymer Tiger Woods v Francesco Molinari