Chadli had only recently returned from a long layoff, but the 28-year-old played two games for Belgium in the space of four days during the international break.
In his first training session back in the West Midlands, Chadli picked up an injury which will keep him out "for the foreseeable future", and Pulis has admitted that he is disappointed with the situation.
The 59-year-old told reporters: "We are very, very disappointed. Chadli has come back, [having] played two games for Belgium.
"We are disappointed he played the 90 minutes [in both], and has ended up getting an injury yesterday in training. That is going to keep him out for the foreseeable future.
"Chadli missed the pre-season, we played him in a game and a half, he did not feel 100% fit, and him going off to play for Belgium, and play two games in a short period of time, has unfortunately caught him out."
Pulis last selected Chadli for the away trip to Southampton on October 21.