Sheffield United's board have issued a strongly-worded statement condemning their fans' behaviour during and after Saturday's home defeat at the hands of Norwich City.
According to the statement, "supporters were injured, objects were thrown onto the pitch and high-profile Norwich officials were abused" during the Championship encounter at Bramall Lane.
The Blades, who face city rivals Sheffield Wednesday at Hillsborough on Sunday, have been reported to the Football Association and could face sanctions, including financial penalties.
Further South Yorkshire Police investigations are said to be ongoing and Sheffield United's CCTV footage is being used to support the attempts to identify individuals.
The United board wrote: "We simply will not tolerate the unsportsmanlike and illegal conduct of a few to spoil things for the large majority who come along to simply watch and enjoy a football game on a Saturday afternoon. At Bramall Lane family values are paramount.
"Violence in any form is unacceptable at Bramall Lane. The club invests significant sums and management time in assuring the safety of our fans and guests and we shall redouble our efforts to ensure that no supporters are injured and no attendees are obnoxious or abusive towards the directors and officials of our fans and guests, such as Norwich. We'd like to assure our fans and supporters that the staff and management of Sheffield United, in co-operation with South Yorkshire Police, will make every effort to assure a safe environment for the derby at the weekend.
"To a degree all in football expect good natured comments at a game - it is part of the culture of football. Enthusiasm, however, can never be an excuse for violence. We are working with the police in coordinating a root and branch approach to identify those supporters who are tarnishing the name of Sheffield United and putting football in a very poor light.
"At a time when we are aiming to have fewer security-related incidents, these individuals seem concerned only with their selfish desire to ruin the wonderful Bramall Lane experience for everyone else.
"We want this element removed from our support so if that means other fans reporting incidents of unacceptable behaviour then let us know. We will issue life bans and impose other tough measures to deal with these fans."
Additionally, the club is continuing to work with Cleveland Police following the August fixture at Middlesbrough, which saw violence break out at the end of the match.