The 28-year-old had to be replaced midway through the first half of Madrid's 3-0 win over Las Palmas in La Liga on Saturday night, and he was seen in tears on the bench.
Nacho later confirmed that he had picked up a hip injury, and it had been thought that the versatile defender would miss the Juventus clash on Tuesday night.
Nacho has made 36 appearances for Madrid in all competitions during the 2017-18 campaign.
Goalkeepers: Navas, Casilla, Luca Zidane
Defenders: Carvajal, Vallejo, Ramos, Varane, Nacho, Marcelo, Theo, Achraf
Midfielders: Kroos, Modric, Casemiro, Llorente, Asensio, Isco, Kovacic, Ceballos
Attackers: Ronaldo, Benzema, Bale, Vazquez, Mayoral