Jorginho and Riccardo Montolivo have been left out of Italy's squad for Euro 2016 this summer.
Manager Antonio Conte named a preliminary 30-man squad, but cut seven players to get down to the final 23-man roster for the tournament in France.
Conte is already without injured pair Claudio Marchisio and Marco Verratti, in addition to the dropped Andrea Pirlo, but he has opted to leave Jorginho and Montolivo at home, with the latter having struggled with a calf problem recently.
Thiago Motta has suffered with a similar injury but is included in the squad alongside Daniele de Rossi, who came through Sunday's friendly victory over Scotland unscathed despite concerns over an Achilles injury.
Italy get their campaign underway against Belgium on June 13, before subsequent matches against Sweden and the Republic of Ireland.
Italy squad for Euro 2016:
Goalkeepers: Gianluigi Buffon (Juventus), Federico Marchetti (Lazio), Salvatore Sirigu (Paris Saint-Germain)
Defenders: Andrea Barzagli (Juventus), Leonardo Bonucci (Juventus), Giorgio Chiellini (Juventus), Matteo Darmian (Manchester United), Mattia De Sciglio (AC Milan), Angelo Ogbonna (West Ham)
Midfielders: Federico Bernardeschi (Fiorentina), Antonio Candreva (Lazio), Daniele De Rossi (Roma), Stephan El Shaarawy (Roma), Alessandro Florenzi (Roma), Emanuele Giaccherini (Bologna), Marco Parolo (Lazio), Stefano Sturaro (Juventus), Thiago Motta (Paris Saint-Germain)
Forwards: Eder (Inter), Ciro Immobile (Borussia Dortmund), Lorenzo Insigne (Napoli), Graziano Pelle (Southampton), Simone Zaza (Juventus)