The hosts suffered a defeat to Always Ready in their opening game, while the Venezuelan side started their campaign with a 3-2 win over Olimpia.
Internacional kicked off their Copa Libertadores campaign with a disappointing defeat last Tuesday, as they travelled to Bolivia to take on Always Ready.
Miguel Angel Ramirez's side did bounce back in style in their latest domestic game on Sunday though, as they recorded an eye-catching 5-0 win at home to Esportivo.
That sealed their second-placed finish in the Campeonato Gaucho, confirming their qualification for the semi-finals.
For now, Ramirez's men will focus on the Copa Libertadores, as they look to bounce back from the opening defeat and put a win on the board to keep their chances of qualification alive.
Their opponents boosted their qualification bid with a 3-2 victory at home to Olimpia last Tuesday.
However, they suffered a shock defeat in their most recent league outing, as goals from Luis Castro, Yanowsky Reyes, Junior Paredes, Heiderber Ramirez and Elias Zimnavoda condemned them to a 5-0 defeat away at Zulia in the Venezuelan top flight.
While that was an alarming result, Tolisano did rest several key players for that game, firmly placing his priority on Wednesday's Copa Libertadores match, as they look to book their place in the knockout stage.
Miguel Angel Ramirez has rotated his goalkeeping options this season, but he seems to have stuck with Marcelo Lomba now, and he is expected to feature again on Wednesday.
Forward Yuri Alberto will be hoping for a starting spot, having found the net in the recent 5-0 victory.
He will have to battle for a starting place with Thiago Galhardo, who recently netted a hat-trick in a league win over Aimore.
While Deportivo Tachira winger Esli Garcia was shown a red card in the league defeat to Zulia, he will not be suspended for this game, with the ban instead applying to the next domestic match.
Ramirez could look to stick with the same lineup that sealed the 3-2 victory over Olimpia, with Lucas Emanuel Gomez and Edgar Perez Greco expected to lead the line.
Freddy Gondola will offer an attacking threat from the left-hand side, having found the net in that last win.
Internacional possible starting lineup:
Lomba; Da Fonseca, Gabriel, Cuesta, Moises; Dourado, Edenilson; Palacios, Mauricio, Vidal; Galhardo
Deportivo Tachira possible starting lineup:
Varela; Camacho, Trejo, Vivas, Granados; Hernandez, Flores, Cova, Gondola; Gomez, Greco
We say: Internacional 2-1 Deportivo Tachira
Despite the contrasting starts of the two sides, the Brazilian outfit have more than enough to come away from this clash with three crucial points.
A 5-0 win last time out will raise their confidence, and they come into this game knowing a result is required to keep their hopes of qualification alive.