Southgate does not have any concerns from the side that started Sunday's 1-0 win over Croatia, though, and it would not be a surprise to see the same XI sent onto the field.
Harry Maguire is making steps forward in his recovery from an ankle injury and has now declared himself fit, but it seems unlikely that the Manchester United captain will return for this match, while Jordan Henderson is not expected to come into the XI, as the Liverpool skipper manages his recovery from a long-term groin issue.
Kalvin Phillips was outstanding against Croatia and should again start alongside Declan Rice and Mason Mount in midfield, while Raheem Sterling and Phil Foden may again join Harry Kane in attack despite the number of other options in that area of the field.
Tyrone Mings should also have done enough to keep his spot in the middle of the defence, having impressed alongside John Stones in England's tournament opener at Wembley, with Maguire potentially in line to make his return against Czech Republic.
England possible starting lineup: Pickford; Walker, Stones, Mings, Shaw; Phillips, Rice, Mount; Sterling, Kane, Foden