The Scottish champions make the trip to Camp Nou in Group H knowing that they have no chance of reaching the last 16 of the competition.
However, Pukki believes that the Hoops have what it takes to cause an upset against the Spanish giants.
"We can win it and we all want to win it," the Finland international told Celtic's official website.
"We want to show in this match that we can win games in the Champions League. As much as we wanted to still be in the tournament at this stage we have nothing to lose now.
"We can go into the game with no pressure, and the Camp Nou is one of the best places to play."
Pukki has scored two goals in 16 games for Celtic following his summer move from Schalke 04.