The running back injured his hamstring during the AFC South side's game against the Houston Texans last week, while Shorts has missed two practices this week with a groin injury which has hampered him throughout the season.
The head coach told reporters: "I think they're both very questionable, very questionable. We have our fingers crossed on both of them.
"We're trying to keep Ace [Sanders] locked in at his position and not move him around. Mike Brown gives us flexibility to play all the positions."
Shorts added: "It's a 'feel' thing. I've been dealing with it all year, it's just a matter of it not getting better. It's getting worse. We'll see how it feels [today]. If I'm able to play, I'm playing."
The Jaguars have won four of their last five games.