Reports had linked the Italian midfielder with a move away from the Stadio Olimpico following recent comments from Baldini that suggested that Roma would consider offers for the 29-year-old.
However, Baldini has now moved to clarify his comments.
"Maybe I was a bit careless and that has led to this situation, but Roma does not want to sell De Rossi," he told Rai Sport.
"I answered a question about what I would do if offers came in. But once and for all, we are not interested in selling."
English champions Manchester City are said to be interested in signing De Rossi.