Galatasary beat Madrid 3-2 last April in the second leg of their Champions League quarter-final clash, but the Spanish giants still prevailed 5-2 on aggregate.
Jose Mourinho was in charge of Madrid that night but he has since been replaced by Ancelotti, who is under pressure to deliver instant success in Europe's elite club competition.
In the Turkish Super League, Gala have failed to win any of their last three league games, but Didier Drogba did get his first goal of the campaign last Friday as Fatih Terim's side drew 1-1 against Antalyaspor.
Madrid have included Gareth Bale in their squad for the Group B opener following his scoring debut in a 2-2 draw at Villareal in La Liga last weekend, but the Welshman is a doubt to start on Tuesday as he bids for full fitness.
Real Madrid: WWWWD
Possible starting lineups
Galatasaray: Muslera, Eboue, Chedjou, Nounkeu, Riera, Felipe Melo, Inan, Amrabat, Sniejder, Yilmaz, Drogba
Real Madrid: Lopez, Carvajal, Ramos, Pepe, Marcelo, Khedira, Modric, Isco, Ronaldo, Bale, Benzema
Sports Mole says: 1-2
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