The Copa Libertadores holders travel to Buenos Aires to face the winners of the Copa Sudamericana, with both teams looking to lift the trophy for the first time.
Defensa y Justicia claimed their first major piece of silverware in January when they won the Copa Sudamericana, beating fellow Argentinian outfit Lanus 3-0 in the final.
The Hawks only established themselves as a top division side in 2014 and since then have gradually improved, becoming one of the strongest sides in Argentina.
Their league form this campaign, however, has been inconsistent as they have won three and lost three of their opening eight matches and as a result sit sixth in Group B of the Primera Division, two points behind the top four.
Defensa lost 2-1 away to Argentinian giants Boca Juniors last weekend. Despite Walter Bou giving the Hawks the lead, goals from former West Ham United pair Carlos Tevez and Mauro Zarate secured all three points for the hosts.
Sebastian Beccacece, who replaced Hernan Crespo as head coach in February, will be aware that his side face another difficult challenge on Wednesday but has belief that his players can take a positive result into the second leg next week.
Palmeiras celebrated the treble in 2020, not only lifting their second Copa Libertadores trophy, but also winning the Copa do Brasil and the Campeonato Paulista.
The Palestra Alviverde also came fourth in the FIFA Club World Cup, losing to Egyptian side Al Ahly in the third-place playoff.
However, they will be hoping to improve their league form this campaign, after finishing in seventh place last season, 13 points behind champions Flamengo.
Palmeiras are known as one of the most successful Brazilian football clubs in history and claiming more silverware in 2021 is at the top of their wishlist once again.
Since the Recopa Sudamericana was formed in 1989, Palmeiras have surprisingly never played in the competition before, but head coach Abel Ferreira will believe that his squad have a great chance of adding another trophy to their cabinet this month.
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Defensa used a 3-4-2-1 formation in the loss to Boca Juniors last weekend and they could stick with the same system on Wednesday.
Striker Bou, who has scored five goals in eight appearances in 2021, is set to lead the line in attack.
Defensa y Justicia possible starting lineup:
Unsain; Rodriguez, Frias, Meza; Breitenbruch, Larralde, Loaiza, Benitez; Martinez, Pizzini; Bou
Palmeiras possible starting lineup:
Silvestre; Mayke, Gomez, Renan, Esteves; Danilo, De Paula; Rony, Lima, Scarpa; Elias
We say: Defensa y Justicia 0-1 Palmeiras
Both Defensa y Justicia and Palmeiras will be keen to ensure that they keep this tie alive and avoiding defeat would give both sides confidence heading into the second leg next week.
This first-leg encounter is set to be a cagey affair, but we can see the visitors securing a narrow victory at the Estadio Norberto Tomaghello.