Gary Bowyer's side have won three league games in a row to move within eight points of the Bees, who occupy the final top-six place with nine games of the season remaining.
Rovers are still in the FA Cup as well and with their quarter-final replay against Liverpool not for another three weeks, Bowyer could go all out in a game which is vital for any outside playoff hopes.
Rumours linking Rhodes with a loan move away have quietened and following his brace in Saturday's 3-1 win at Charlton Athletic, the Scotland international has now scored six goals in his last six starts.
Rovers win at The Valley was an impressive one considering that Charlton had won their previous three games, but it came at a cost with Doneil Henry, Lee Williamson and Craig Conway suffering injuries.
While Blackburn are flying in the league, Brentford are just about holding on to their place in the playoffs following a run of one win in four games.
Saturday's 2-1 defeat at home to nine-man Cardiff City saw Mark Warburton's side fall seven points behind the pacesetting trio of Bournemouth, Watford and Middlesbrough and a playoff berth is now their only realistic way of getting promoted.
Both Cardiff goals would have been very avoidable from Warburton's point of view and the Bees have now made as many errors leading directly to a goal (9) as anyone else this season.
Blackburn have picked up 22 out of a possible 24 points in their last eight home league games against Brentford, but the reverse went in the Bees' favour as they won 3-1 in mid-December.
Form in Championship: DLDWWW
Form in all competitions: LDWDWW
Possible starting lineup: Steele; Henley, Kilgallon, Baptiste, Spurr; Taylor, Williamson, Cairney, Marshall; Gestede, Rhodes
Recent form: WWLWDL
Possible starting lineup: Button; Odubajo, Tarkowski, Dean, Bidwell; Douglas, Diagouraga; Jota, Pritchard, Dallas; Gray
Sports Mole says: 2-2