The Yorkshire club came close to snatching a point at high-flying Leicester City on Saturday and the performance may inject more confidence into the team ahead of the match against Nigel Adkins's Royals.
Meanwhile, Adkins's reading have won three on the spin and are unbeaten in five, a run that has seen them leap into the playoff spots.
These two sides last met back in March 2012 when Reading netted four second-half goals to brush aside the Tykes.
Possible starting lineups
Barnsley: Butland; Wiseman, Cranie, Mvoto, Kennedy; Cywka, Dawson, Perkins, Fox, Jennings; O'Grady
Reading: McCarthy; Gunter, Morrison, Pearce, Bridge; McCleary, Baird, Guthrie, Robson-Kanu; Pogrebnyak, Le Fondre
Sports Mole says: 0-2