Williams took the first set 6-3 after breaking her Danish opponent on three occasions.
The American also broke twice in the second set, meanwhile, in addition to holding each of her four serves to claim the title 6-3 6-3.
It is Williams's 18th Grand Slam and sixth US Open title, but Wozniacki's wait for a Grand Slam continues.
Read how it all unfolded in Sports Mole's game-by-game live commentary of the action below.
9.11pmHello all! The 2014 women's US Open draws to a close this evening as Serena Williams faces Caroline Wozniacki in New York. Williams, who is the top seed and defending champion, is the favourite to overcome 10th-seed Wozniacki, but as we saw in the men's semi-finals yesterday, surprises can happen. Just a reminder that the match is due to start at 9.30pm UK time.
9.14pmThe pair met in the Western & Southern Open just last month, with Williams winning in three sets to book her spot in the final of that particular competition. Williams also beat Wozniacki in the quarter-finals of the Rogers Cup in August. Overall, the American has won eight of the nine meetings between the two players, with Wozniacki's only win coming in 2012.
9.17pmWilliams, 32, is attempting to win her 18th Grand Slam in New York. During an incredible career, she has won the Australian Open five times, the French Open twice, Wimbledon five times and the US Open five times – including each of the last two tournaments.
9.21pmWozniacki, meanwhile, is still waiting for her first Grand Slam. The 24-year-old reached the final of the US Open in 2009, but that is her only final to date. Elsewhere, the Dane has made the semi-finals of the Australian Open and quarter-finals of the French Open.
9.24pmIn terms of Williams's route to the final, the American beat Taylor Townsend, Vania King, Varvara Lepchenko and Kaia Kanepi in the early rounds, before overcoming Flavia Pennetta in the quarter-finals. Her spot in the final was secured after overcoming Ekaterina Makarova 6-1 6-3 in a fairly routine semi-final clash on Friday.
9.27pmWozniacki, meanwhile, beat Magdalena Rybarikova, Aliaksandra Sasnovich, Andrea Petkovic in the early rounds, before beating Maria Sharapova in the round of 16. Sara Errani was then dispatched in the quarter-finals, before Shuai Peng was forced to retire during their semi-final clash on Friday.
9.33pmWilliams and Wozniacki are good friends off the court, but there will be no room for sentiment in this match. Not when the US Open is up for grabs and the tasty pay packet that comes with it.
9.36pmAs expected, it is a beautiful day in New York City. The sun is shining and the crowd is expectant. The match is due to start in the next five minutes, although this is not set in stone!
9.39pmJust a reminder that Williams is going in search of her sixth US Open this evening and her 18th Grand Slam overall. Wozniacki, however, is yet to win a Grand Slam despite her standing in the women's game.
9.45pmThis is Serena's manor these is no question about that, she just loves this competition. I cannot see Wozniacki upsetting causing a shock this evening and I am backing Williams to win in two sets.
9.46pmHere we go then. Both players join us on court...
9.48pmWilliams has won the toss and the American chooses to serve first. It is almost time.
9.50pmBoth players are just knocking up ahead of the start of this contest. Williams looks incredibly focused, while Wozniacki looks slightly more relaxed. Wozniacki simply has to start well this evening.
9.53pmAnd then there were two. The stage is set for the conclusion of the women's 2014 US Open. In terms of the weather, there is no wind in New York, which will certainly suit Williams's style of play.
9.55pmTime has been called. This is it. It will be Williams to serve first...
Williams 1-0 *WozniackiWilliams moves 15-0 ahead when Wozniacki fires long in the first point of the final, but the American then hits a forehand wide for 15-15. A forehand pass moves the defending champion 30-15 ahead, but Williams then nets for 30-30. Wozniacki has break point when Williams hits another one long, but an ace saves Williams for deuce. Williams then comes to the net to move onto advantage, before holding with a smash at the net.
Williams* 2-0 WozniackiBREAK! A stunning forehand allows Williams to move 0-15 ahead when Wozniacki cannot return, but Williams then nets for 15-15. A double-fault from Wozniacki allows Williams to move 15-30 ahead, but then the American hits a powerful forehand long for 30-30. A second double-fault for Wozniacki allows Williams to move onto 30-40, but once again, Williams hits long for deuce. The defending champion responds in the next point with a stunning forehand and breaks with a forehand down the line.
Williams 2-1 *WozniackiBREAK! Williams moves 15-0 ahead when Wozniacki hits a forehand long, before another ace moves the American 30-0 ahead. Williams comes to the net in the next point, but can only hit the net to allow Wozniacki onto the board. Williams then hit one long for 30-30, before netting once again to hand Wozniacki break point. The Dane takes it when Williams finds the side-court with a forehand.
Williams* 3-1 WozniackiBREAK! Williams moves 0-15 ahead when Wozniacki hits a forehand long of the baseline, before whipping a forehand past Wozniacki for 0-30. The 10th seed responds with a powerful serve which proves non-returnable, before Williams nets for 30-30. Wozniacki's first ace then moves her 40-30 ahead, but Williams ties it at deuce with a stunning backhand. Wozniacki then hits one wide to hand Williams advantage, but the American then fires long for deuce two. Eventually Williams breaks after deuce three when Wozniacki hits the net.
Williams 3-2 *WozniackiBREAK! Williams nets in the first point to hand her opponent 0-15, before clipping one wide for 0-30. Williams then double-faults to hand Wozniacki 0-40, but responds with an ace for 15-40. Wozniacki does break in the next point, however, when Williams double-faults once more.
10.18pmAny danger of anyone holding their serve here?
Williams* 4-2 WozniackiBREAK! Wozniacki moves 15-0 ahead when Williams fires a forehand wide, but the American responds in the next point with a passing shot. Williams then hits one down the line to move onto 15-30, before Wozniacki nets to hand Williams 15-40. Williams hits Wozniacki's next serve long for 30-40, but breaks when Wozniacki nets.
Williams 5-2 *WozniackiBrilliant start from Williams as she hits a superb forehand for 15-0, but the American then clips wide to hand Wozniacki 15-15. Williams has 30-15 in the next point when Wozniacki hits long of the baseline and the Dane follows suit in the next point for 40-15. Finally we have a hold of serve when Wozniacki nets once more.
Williams* 5-3 WozniackiWilliams hits another forehand winner to move 0-15 ahead, but the American then fires long for 15-15. Williams then places one long of the baseline for 30-15, but Wozniacki double-faults in the next point for 30-30. Williams hits a forehand long in the next point to hand Wozniacki 40-30, but the Dane then follows suit for deuce. Wozniacki again hits the net to hand Williams set point, but the American then hits one wide for deuce two. Eventually Wozniacki holds when Williams again hits the net.
SET! Williams 6-3 WozniackiSET! Brilliant from Wozniacki as she hits a forehand winner to move 0-15 ahead, but Williams responds with a powerful serve for 15-15. Williams then moves onto 30-15 when a deep stroke from Wozniacki drops wide of the court, before another strong serve moves the American 40-15 ahead. The set is secured with a brilliant forehand.
Williams* 6-3 1-0 WozniackiBREAK! Powerful start from Williams as she forces Wozniacki to hit the net in the first point of the second set, but the American then hits a routine forehand long of the baseline for 15-15. Williams comes to the net in the next point for 15-30, before moving 15-40 ahead with help from the net. The break arrives when Wozniacki nets once more.
Williams 6-3 2-0 *WozniackiWilliams starts with a sensational ace to move 15-0 ahead, before Wozniacki nets for 30-0. Another ace brings 40-0, before Williams holds when Wozniacki hits long of the baseline.
Williams* 6-3 2-1 WozniackiWozniacki has 15-0 when Williams hits one onto the side-court, but Williams responds with an overhead smash. Wozniacki takes the next point with a forehand down the line, but Williams answers straight back as Wozniacki hits the net for 30-30. A powerful serve allows the Dane to set up game point, but deuce arrives when Williams smashes a backhand across the court. The pair then exchange points before arriving at deuce once again, but eventually Wozniacki holds when Williams fires wide.
Williams 6-3 3-1 *WozniackiWilliams starts the game with a double-fault to hand Wozniacki 0-15, but responds with a forehand passing shot. Wozniacki then hits two forehands long of the baseline to hand Williams 40-15 and the American holds with a sensational volley.
Williams* 6-3 3-2 WozniackiSensational tennis from Williams once again as she dominates the first rally of the game for 0-15, but the American then hits the net for 15-15. Williams also nets in the next two points to hand Wozniacki 40-15, before the Dane holds with an ace.
Williams 6-3 4-2 *WozniackiWilliams starts with another ace to move 15-0 ahead, but then fires long for 15-15. Another ace allows the American to regain the lead, before Wozniacki fails to return another serve in the next point. Williams holds with yet another sensational serve.
Williams* 6-3 4-3 WozniackiWilliams nets in the first point to hand Wozniacki 15-0, before firing long for 30-0. Williams then hits the net as Wozniacki moves onto 40-0 and the Dane holds with a superb ace.
Williams 6-3 5-3 *WozniackiWilliams moves 15-0 ahead when Wozniacki fires wide, before the Dane nets to hand Williams 30-0. Williams then hits a backhand wide to allow her opponent onto the board, before Williams nets for 30-30. The American responds with a stunning serve into the body, however, before holding with a fine forehand.
SET! Williams 6-3 6-3 WozniackiGAME, SET AND MATCH! Williams has 0-15 when Wozniacki fires long of the baseline, but the Dane responds with a brilliant passing shot to move onto 15-15. The point of the final arrived next - with Williams claiming it after 26 shots. Williams then has 15-40 when the American produces a brilliant forehand and she wins it when Wozniacki fires long.
11.12pmSERENA WILLIAMS WINS THE 2014 WOMEN'S US OPEN
11.14pmWow, just wow. That was some performance from Williams. What a sensational display. Wozniacki just could not match the power of the American. It is Serena's 18th Grand Slam title and sixth US Open, incredible.
11.16pmWe are just about ready for the trophy presentation here...
11.18pm"First of all congratulations Serena, you really deserved it. You are an unbelievable champion and friend and you owe me a drink later," Wozniacki has just told the crowd on court.
11.19pmWozniacki also thanks the crowd, the sponsors and volunteers for their role in the US Open. Classy from the tenth seed there.
11.20pm"It was a really wonderful feeling (to win it). It was a wonderful finish, I would like to say congrats to Caroline, she is such a great person and I really am so happy for her. I know you will be winning very soon. Thanks to the crowd," said an emotional Williams.