Johnstone's Paint Trophy debutantes Wolverhampton Wanderers have been handed a home draw against fellow West Midlanders Walsall in round one.
Wolves, who are competing in the third tier of English football for the first time since 1989 this season, will host the Saddlers in the week commencing September 2.
Other standout ties include Scunthorpe United hosting Sheffield United, and Portsmouth travelling to Torquay United.
Meanwhile, 16 clubs, including Preston North End, Mansfield Town, Newport County and Coventry City, have received byes to the second round.
Draw in full:
Northern section:
Wolverhampton Wanderers vs. Walsall
Hartlepool United vs. Bradford City
Scunthorpe United vs. Sheffield United
Notts County vs. Burton Albion
Port Vale vs. Bury
Tranmere Rovers vs. Fleetwood Town
Crewe Alexandra vs. Accrington Stanley
Shrewsbury Town vs. Oldham Athletic
Byes:
Carlisle United, Morecambe, Preston North End, Rochdale, Chesterfield, Mansfield Town, Rotherham United, York City
Southern section:
Cheltenham Town vs. Plymouth Argyle
Exeter City vs. Wycombe Wanderers
Bristol City vs. Bristol Rovers
Torquay United vs. Portsmouth
Brentford vs. AFC Wimbledon
Gillingham vs. Leyton Orient
Dagenham & Redbridge vs. Colchester United
Milton Keynes Dons vs. Northampton Town
Byes:
Crawley Town, Newport County, Oxford United, Swindon Town, Coventry City, Peterborough United, Southend United, Stevenage
