A late defeat in France last weekend saw England get their campaign off to a losing start.
However, Twelvetrees is confident that the team can pick up their first win of the tournament if they stay focused at Murrayfield.
"We're fully aware now of what we have to do for the rest of the championship - win four games and do it as well as we can," he is quoted as saying by Sky Sports News.
"This week is huge for us to get back on the road of winning and that's our focus. If we get the simple things well and do them right I think it will take care of itself."
Scotland head into the match on the back of a 28-6 defeat to Ireland on the opening weekend.