A 26-24 defeat to France in Paris last weekend saw England get their campaign off to a losing start.
Despite the visitors being favourites for their upcoming match in Edinburgh, Rowntree believes that the Scots will be in a determined mood after losing their opener to Ireland.
"It was a very down dressing room in Paris and rightly so, but it's our job as coaches to get the players back up very quickly because we have another big challenge this week," he is quoted as saying by Sky Sports News.
"Scotland will be fed up themselves after losing to Ireland and will be waiting for us at Murrayfield. We've had a good look at that game and they'll fancy their chances against us."
England have beaten Scotland in each of the last four meetings between the sides.