Ashton has grabbed a place back in the squad, which has pushed Charlie Sharples over to the left, following his one-match suspension.
Meanwhile, Australia head into Saturday's match on the back of a 33-6 defeat to France.
England team to play Australia:
Alex Goode, Chris Ashton, Manusamoa Tuilagi, Brad Barritt, Charlie Sharples, Toby Flood, Danny Care, Joe Marler, Tom Youngs, Dan Cole, Tom Palmer, Geoff Parling, Tom Johnson, Chris Robshaw, Thomas Waldrom
Replacements: David Paice, David Wilson, Mako Vunipola, Joe Launchbury, Tom Wood, Ben Youngs, Owen Farrell, Mike Brown