Enoch Barwuah, who is the biological sibling of the Italy international, was called offensive names and subjected to "monkey noises" during a derby match while playing for Vallecamonica.
Enoch has revealed that Balotelli was also mentioned in the heckling during the Eccellenza League match against Darfo Boario in the Brescia area.
"They called me a dirty n****o, made monkey noises and even brought my brother Mario into it," Football Italia quotes Enoch as saying.
"In all honesty I didn't understand why they were so focused on me. At the start of the game I was annoyed, as the chants were irritating, but then I pretended not to hear them.
"It's not the first time, although I had not been insulted like this for a long while. People who behave this way do not even deserve me wasting time to think about them."
Enoch later posted an image to Instagram with the message: "Let's stay calm."