The Australian has jumped to the number one slot after spending 38 weeks as second, behind Woods, who held the top ranking for 15 months.
This season the 33-year-old finished 14th at the Masters in Augusta and recorded a third-placed finish at the Arnold Palmer Invitational, but Scott is yet to win a USPGA Tour event this year.
Chief executive of the PGA of Australia Brian Thorburn told their official website: "This is another outstanding achievement for Adam Scott. The Australian golfing community is very proud of Adam and we look forward to watching his progress throughout the year."
Scott became the first Australian to win the Masters last year.