The 38-year-old American has been suffering with a back injury for the past few months.
The four-time champion has now revealed that he will not be able to participate at Augusta National Golf Club after undergoing successful surgery to fix a pinched nerve.
"After attempting to get ready for the Masters, and failing to make the necessary progress, I decided, in consultation with my doctors, to have this procedure done," Woods told his official website.
"I'd like to express my disappointment to the Augusta National membership, staff, volunteers and patrons that I will not be at the Masters. It's a week that's very special to me. It also looks like I'll be forced to miss several upcoming tournaments to focus on my rehabilitation and getting healthy.
"I'd also like to thank the fans for their support and concern. It's very kind and greatly appreciated. This is frustrating, but it's something my doctors advised me to do for my immediate and long-term health."
The 2014 Masters gets underway on April 10.