Europe captain Paul McGinley has selected Graeme McDowell to open up his side's bid to retain the Ryder Cup in Sunday's singles action.
The Northern Irishman, who has partnered Victor Dubuisson to two points on Friday and Saturday, will play American rookie Jordan Spieth.
Dubuisson will tee off last against Zach Johnson as the hosts look for the four points that they require to retain the trophy that they won in Medinah two years ago.
Henrik Stenson is out second against Patrick Reed, Rory McIlroy follows against Rickie Fowler while form player Justin Rose will play Hunter Mahan.
The other stand-out tie sees home favourite Stephen Gallacher face Phil Mickelson.
Full lineup
Graeme McDowell vs. Jordan Spieth Henrik Stenson vs. Patrick Reed Rory McIlroy vs. Rickie Fowler Justin Rose vs. Hunter Mahan Stephen Gallacher vs. Phil Mickelson Martin Kaymer vs. Bubba Watson Thomas Bjorn vs. Matt Kuchar Sergio Garcia vs. Jim Furyk Ian Poulter vs. Webb Simpson Jamie Donaldson vs. Keegan Bradley Lee Westwood vs. Jimmy Walker Victor Dubuisson vs. Zach Johnson