Stokes has just spent seven days in court after an incident at a Bristol nightclub, but the all-rounder was handed an immediate recall to the squad having been found not guilty of affray.
A decision to put Stokes in contention for an outing at Trent Bridge has divided opinion, but Root has announced that the Durham player will play instead of Sam Curran.
At a press conference, Root told reporters: "He is desperate to get out there and wants to put in performances. I have known Ben from being really quite young and I feel very confident he will be able to go out and put in a performance."
England take a 2-0 lead into the third match of the best-of-five series.