Pietersen's absence was expected after he was left out of the England squad for the third Test against South Africa after the controversy surrounding texts he sent to members of the Proteas team.
The 14-man one-day squad consists of players that featured in recent victories over West Indies and Australia.
The 15-man World Twenty20 will be skippered by Stuart Broad, with Michael Lumb and Luke Wright returning to the squad for the first time since June 2011.
England NatWest ODI Series squad:
A.Cook (c), J.Anderson, J.Bairstow, I.Bell, R.Bopara, T.Bresnan , J.Dernbach, S.Finn, C.Kieswetter, E.Morgan, S.Patel, G.Swann, J.Trott, C.Woakes
World T20 Squad:
S.Broad (c), J.Bairstow, R.Bopara, T.Bresnan, D.Briggs, J.Buttler, J.Dernbach, S.Finn, A.Hales, C.Kieswetter, M.Lumb, E.Morgan, S.Patel, G.Swann, L.Wright