India are considered by the bookmakers to be the favourites to emerge victorious in Birmingham, while England have never won a 50-over title in 16 attempts.
However, despite seemingly having the odds stacked against them, Cook has insisted that his players are confident of breaking their duck.
"The day before a big final [and] you can sense that excitement," he said. "The guys are just raring to go. We're ready to play. I'm fully confident in our guys that we can upset India here on Sunday.
"It is very important for us - one of our major goals this summer was to try and win the Champions Trophy. Not only will it break that duck of 38 years but also prepare well for the World Cup, if you're looking long-term.
"The lads, in the last two games under pressure, delivered two excellent performances. I can see no reason why we can't do it. They are unbeaten and have played some very good cricket, but our record against India last time we played them in England is good. We can certainly take a lot from that."
England have previously lost four finals over the shorter format of the game.