Nedovic picked up what was originally thought to be a stress fracture while representing Serbia against Italy in a warm-up fixture, but the Serbian team doctor has now confirmed that the star has damaged his fifth metatarsal.
"After last medical check, there is no doubt," said Nedovic's agent Misko Raznatovic via Twitter.
"Nemanja Nedovic, unfortunately, will miss World Cup in Spain. He needs to travel to USA, [on Saturday]."
The World Cup is due to get underway at the end of this month.