England's men had won gold less than 20 minutes prior to the race, and a team of Asha Philip, Dina Asher-Smith, Bianca Williams and Lorraine Ugen ensured that they would also be standing atop the podium with a national-record time.
A blistering second leg from Asher-Smith took England into the driving seat at the halfway stage of the race, and Williams increased that lead going into the home straight.
It looked at one stage as though Ugen might be caught by the Jamaicans on the inside lane, but she held off the challenge of double Olympic champion Elaine Thompson to claim gold by just 0.06s.
A Nigerian team including Blessing Okagbare - double gold medallist in 2014 - and Oluwatobiloba Amusan, who won gold in the 100m hurdles final last night, crossed the line in third place to claim bronze ahead of Trinidad and Tobago.
There was disappointment for host nation Australia, meanwhile, as they were disqualified, with Ghana and Cameroon completing the standings.