Great Britain's rhythmic gymnasts will not be participating at this summer's Olympics after failing to meet the qualifying standard set by their governing body.
The team, whose hopes rested fully on Tuesday's performance, fell 0.273 marks short of the 45.223 target set.
An appeal was raised to increase the score but it was rejected.
British Gymnastics performance director Tim Jones told BBC Sport: "We went through a process with the British Olympic Association (BOA) of agreeing a standard we felt would show credible performance.
"It would have been a score that gave us a platform to launch rhythmic into the next four years, but there wasn't any leeway. They will not be nominated [for London 2012]."
The Games begin on July 27.