Spurs have travelled to Turin having won a Champions League group which included Real Madrid and Borussia Dortmund, but Juventus head into the first leg of the last-16 tie having conceded just one goal in 15 matches in all competitions.
Kane has been in free-scoring form, but the England international has admitted that he faces one of his biggest tests against a backline led by Giorgio Chiellini.
At a press conference, the 24-year-old told reporters: "Yes, it's one of my biggest tests. I always said I wanted to test myself against the best defences and they have one of them."
On Chiellini, the striker added: "He's an amazing defender. When we first played he went in on me and I had a dead leg for ten minutes. He's a fantastic player."
Kane goes into the game with a return of eight goals from his last seven matches in all competitions.