Expectations are a little lower this time around, however, as the Italian side have collected just five points from their opening four Serie A matches and are in need of a boost.
Out: Javier Pastore
Roma will definitely be without Pastore for their Group G opener as the summer signing is struggling with a muscular problem that also kept him out of the 2-2 draw with Chievo at the weekend.
Daniele De Rossi was used from the bench in that most recent league outing, but the defensive midfielder is not expected to start at the Bernabeu, putting extra pressure on Steven N'Zonzi in front of the back four.
Should De Rossi miss out, Lorenzo Pellegrini and Bryan Cristante are likely to retain their spots in a midfield three, with the latter most at risk of dropping out if Di Eusebio can recall his inspirational skipper.
The recent return to fitness of Alessandro Florenzi has provided a welcome boost, meanwhile, and up top the visitors can call upon Edin Dzeko, who netted a hat-trick when Roma put four unanswered goals past Villarreal on a recent visit to Spain.