Both sides go into the match knowing that a defeat would leave their hopes of reaching the last 16 hanging by a thread.
Juve's 49-game unbeaten streak in Serie A was ended by a 3-1 home loss to Inter Milan earlier this month and Marchisio believes that the setback has helped his side to refocus.
He said: "The loss to Inter has rekindled this light, the nastiness, the rhythm that was missing in various matches.
"Against Nordsjaelland we showed the Juventus of last year, that anger, that conviction. Against Chelsea we must play like in the first game in London."
Juve came back from 2-0 at Stamford Bridge to draw 2-2 back in September.