The match, which will be shown live on BT Sport, will kick off the new season, and will be followed the next day by a London double header at Twickenham.
Last season's runners-up Saracens will host Wasps in a 2pm kickoff, which will be shown on BT Sport prior to Harlequins' clash against London Irish.
Wembley will host a match between Saracens and Harlequins on March 28, while Twickenham will play home to a contest between Harlequins and the current champions on December 27.
Premiership opening weekend fixtures:
Friday, September 5
Northampton Saints vs. Gloucester Rugby (7.45pm)
Saturday, September 6
Saracens vs. Wasps (Twickenham, 2pm)
Sale Sharks vs. Bath Rugby (2pm)
Leicester Tigers vs. Newcastle Falcons (3pm)
London Irish vs. Harlequins (Twickenham, 4.30pm)
Sunday, September 7
London Welsh vs. Exeter Chiefs (2pm)