Spurs have won four games in a row in all competitions, while opponents Zagreb head into the first leg on an eight-game winning streak.
Here, Sports Mole rounds up the latest team news for both sides.
Sports Mole's predicted XI: Lloris; Tanganga, Alderweireld, Dier, Davies; Hojbjerg, Ndombele; Lamela, Alli, Bergwijn; Vinicius
Out: Sadegh Moharrami (muscle)
Sports Mole's predicted XI: Livakovic; Ristovski, Lauritsen, Theophile-Catherine, Gvardiol; Ivanusec, Jakic, Orsic; Ademi, Majer; Petkovic