Queen of the South twice came from behind against Morton before claiming a bonus point in their Betfred Cup opener.
Queens warmed up for their home clash with St Mirren by prevailing 4-2 on penalties at Cappielow following a 2-2 draw against their Scottish Championship rivals.
Gregor Buchanan secured the shoot-out triumph to give Queens the edge in the table.
Matthew Aitken struck twice for Rovers, including a penalty opener, but Luke McCowan equalised for Ayr before Walsh and Anderson netted in an action-packed first half.
Zanatta scored an impressive fourth before setting up on-loan Aberdeen striker Anderson to net his second of the night.
Dunfermline recorded a controversial 1-0 win at Dumbarton. Kevin O'Hara netted a 26th-minute spot-kick after Morgyn Neill was penalised for a disputed handball decision.
Impressive first-half strikes from Luke Donnelly and Thomas O'Brien gave Arbroath a 2-1 win at Stirling, who pulled one back through Andy Ryan in the 76th minute.
On-loan Livingston striker Jack Hamilton headed home the opener 70 minutes into his East Fife debut as his team beat Cowdenbeath 2-0. Ryan Wallace made sure of the three points with a brilliant late volley.
Falkirk and Dundee were earlier awarded 3-0 victories after their respective opponents, Kilmarnock and Forfar, were unable to fulfil their fixtures because of Covid-19 cases.