The Reds ran riot in the first leg to race into a 5-0 lead, but late lapses led to Roma pulling two away goals back and giving themselves a glimmer of hope heading into their home second leg.
The match pits the competition's best attack against a defence which is yet to concede a home goal in the Champions League this season, but Liverpool know that they can afford to lose by two goals and still progress through to face Real Madrid in the final.
Here, Sports Mole looks at how Jurgen Klopp's side might line up in the Italian capital.
Out: Joel Matip (thigh), Emre Can (back), Alex Oxlade-Chamberlain (knee), Joe Gomez (ankle)
Doubtful: Adam Lallana (thigh), Trent Alexander-Arnold (knock), Jordan Henderson (knock), Sadio Mane (hamstring)
Liverpool have a host of injury concerns heading into their biggest European match for a decade, with the main problems coming in midfield with Can and Oxlade-Chamberlain both expected to miss the rest of the season.
The Reds could be boosted by the return of Lallana after he was included in the squad, but he is unlikely to be used from the start given his lack of football over the course of the season.
Alexander-Arnold was deployed in a makeshift midfield role at the weekend and picked up a knock along with captain Henderson, but both are expected to recover in time for tonight's match.
Klopp should also have Mane back available after he sat out Saturday's draw with Stoke City due to a minor injury problem, restoring the devastating front three who have scored 28 goals between them in this season's competition.
Mohamed Salah and Roberto Firmino both scored twice in the first leg and will fancy their chances of getting on the scoresheet again against a team who have little option but to throw caution to the wind.
Such was the nature of Liverpool's first-leg performance that Klopp is likely to change as little as possible, with the enforced absence of Oxlade-Chamberlain expected to be the only difference in personnel on the night.
James Milner, meanwhile, just needs one more assist to become the unlikely holder of the record for most in a single Champions League campaign since the new format was introduced in 2003-04.
Click here to read Sports Mole's full preview of tonight's match at the Stadio Olimpico.